TY - JOUR AU - Ghauri, Aymen Owais AU - Arshad, Muhammad Adeel AU - Khalid, Sana AU - Bilal, Muhammad PY - 2021/12/31 Y2 - 2024/03/29 TI - In vitro Anti-ulcer potential of Raphanus sativus L. seeds JF - International Journal of Natural Medicine and Health Sciences JA - IJNMS VL - 1 IS - 1 SE - Articles DO - 10.52461/ijnms.v1i1.742 UR - https://journals.iub.edu.pk/index.php/ijnms/article/view/742 SP - 1-8 AB - <p><em></em></p><p>Background: <em>Raphanus sativus </em>L. is an indigenous plant that is traditionally used to treat peptic ulcer. Objective: This study assessed anti- <em>Helicobacter pylori, </em>antioxidant, and anti-urease activities of <em>R. sativus </em>seeds. Methods: this study targeted in-vitro model. The antioxidant activity of the methanol extract and <em>n</em>-butanol, aqueous, <em>n</em>- hexane and chloroform fractions of <em>R. sativus </em>seeds was determined using DPPH. Moreover, the inhibitory effects of the extract and fractions on the activity of urease and antibacterial activity against <em>Helicobacter pylori</em> were also evaluated. Results: The crude extract as well as its derived <em>n</em>-butanol fraction exhibited higher (IC<sub>50</sub>: 45 and 31 µg/mL respectively) DPPH inhibition activity and inhibition of urease (IC<sub>50</sub>: 75.0 and 55.9 µg/mL respectively) while aqueous extract inhibited <em>H. pylori</em> strongly (MIC<sub>50</sub>: 15 µg/mL).&nbsp;Conclusion: Collectively, our data show that <em>R. sativus </em>extract and fractions possess antioxidant, anti- <em>H. pylori</em> and anti-urease potential. The traditional uses of <em>R. sativus</em> as anti-ulcer, might be due to its anti-urease activity.</p> ER -