International Journal of Natural Medicine and Health Sciences

Isolation and Identification of Bacteriumon DiagnosticPathology Laboratory Hardware

Rehan Latif — 2024-03-31

Background: The main aim of our study is to check the hygiene level of private and government hospital laboratories instruments and to determine Isolation and identification of bacteria present on clinical laboratories instrument (computers keyboard, mouse and telephone).

Methodology: An analytical cross-sectionalstudywas conducted in the tertiary private and government hospital laboratories of Islamabad, Pakistan from 1^stApril to 30^thSeptember 2023. Samples were collected aseptically from various sections of the laboratory using sterilized wet cotton swabs; clean each computer keyboard, mouse, and telephone dipped in distal water and rotated on every corner of the keyboard, mouse, and telephone. For the isolation of organism, samples were inoculated on MacConkey agar for overnight at 37 ⁰C, after overnight incubation bacterial colonies of different shape, size and color were selected. Following the collection of pure colonies, conventional microbiological methods such as Gram staining and biochemical tests were used to identify the organisms. For antibiotic testing, AST was measured using the dick diffusion method Mueller-Hinton Agar (MHA). For statistical analysis, we used MS Excel and SPSS (version no 22) software.

Results: Out of 120, 42 (35.0 %) samples were collected from keyboard, 42 (35.0 %) from mouse and 36 (30.0 %) from telephone. A total of 59 (49.2 %) samples were resulted as STAPH SPP, 40 (33.3 %) samples were MRS, 6 (5.0 %) samples were MRSA, and 15 (12.5 %) samples were found with no growth. The fact that some of these bacteria are highly resistant to routinely used antibiotics is quite concerning. Multidrug resistance was also discovered.

Conclusion: Antimicrobial resistance to most common antimicrobial agents was also found in high numbers. As a result, pathogenic microorganisms on computer keyboards, mouse and telephones are a cause for concern. Because computer keyboards, mouse and telephones provide a surface for colonization, infection control recommendations must focus on proper surface disinfection and hand hygiene, as well as understanding of how to clean such surfaces or disinfect them.

Dengue Fever: Review with insight into Pathogenesis and suggested Treatment with Prevention

Ejaz Rafique — 2024-03-31

Dengue is a chronic viral infectious disease. The disease has been common and prevalent throughout the world with affecting and having major outbreaks in more than a 100 countries. It is a positive sense, RNA virus. There are number of clinical features which are manifested in patients depending on their age and living circumstances that range from being an asymptomatic illness to a severe symptomatic disease that could prove to be hazardous and life-threatening. Though there are a number of methods which are used for the identification of the virus, but the serological methods are considered to be the most effective and accurate tests for the identification of the infecting serotype and later on for the conformation and diagnosis of the disease. Serological tests used are: Complement Fixation test, Hemagglutination Inhibition test, Neutralization test, and ELISA. Though there are a number of drugs prescribed by the physician for the suppression of symptoms and also a vaccine for dengue has also been licensed.

Predisposing factors of Piriformis tightness and its association with low back pain in office workers - a cross sectional study

Rabia Shaheen Khan — 2024-03-31

Background: Piriformis tightness causes restricted range of motion and decreased flexibility. It interferes with the physical and social functioning of people and leads to piriformis syndrome if not diagnosed in initial stages. Objective: To identify the predisposing factors that may cause piriformis tightness and to check its association with low back pain in office workers. Methods: It was a cross-sectional study and convenient sampling technique was used to collect data from the study participants. Sample size was 250 office worker selected through defined inclusion criteria. Selection criteria were people of 26 to 50 years working from at least of 2 years. Seated piriformis test was used to check the presence of piriformis muscle tightness. Visual analogue scale was used for pain assessment and a structured questionnaire was used to check the predisposing factors. After collecting data from study participants it was analyzed through SPSS version 22. Results: Piriformis tightness was found in 76% office workers. About 58% of the study participants reported to work in office for 6 to 8 hours. 88% of workers reported to sit in slouched posture while 78% reported not to have any foot support. Almost 80% of office workers reported work related back pain. Chi square test resulted a significant association of piriformis tightness with low back pain (p<0.05). Conclusion: Piriformis tightness was frequent in office workers. Predisposing factors included prolong working hours, uncomfortable chairs without appropriate foot support, poor posture, and work related back pain. Study also concluded the significant association between low back pain and piriformis tightness.

Food Hydrocolloids: Functional properties, nutritional benefits and future trends

Madiha Khan Niazi — 2024-03-31

Hydrocolloids represent a cornerstone material within the food industry, offering a versatile array of functionalities crucial for product development and enhancement. Their multifaceted roles encompass thickening, gelling, emulsifying, stabilizing, fat replacement, clarification, flocculation, clouding, and even whipping properties. Moreover, their utility extends beyond conventional applications, encompassing taste encapsulation, prevention of crystallization, and the formation of edible films, thus enriching the sensory and structural attributes of food products. Recent insights have illuminated hydrocolloids' burgeoning role in promoting health and wellness, particularly through their capacity to furnish low-calorie dietary fiber. This burgeoning understanding underscores their potential as functional ingredients capable of addressing evolving consumer demands for healthier food options. This review comprehensively explores the latest advancements in hydrocolloid research, delineating their diverse applications as health-enhancing agents in the food industry. By providing an exhaustive account of their utilization, the review aims to serve as a valuable resource for food scientists, technologists, and manufacturers seeking to optimize product formulations and cater to evolving consumer preferences. Given their pivotal role in improving both functional and nutritional attributes, hydrocolloids emerge as indispensable components across various sectors of the food industry. Their versatility and potential for innovation render them invaluable assets, driving advancements in food technology and facilitating the creation of products that meet the diverse needs and preferences of consumers.

Comparison of aerobic exercises and abdominal fascia releasing technique in primary dysmennorhea

Ramiza Khalid — 2024-05-14

The study was to compare and to know the effects of aerobic exercises and abdominal fascia releasing technique to help woman choosing the best treatment between aerobics and abdominal fascia releasing technique. Background: Menstruation has a broad relevance for women in terms of biological, cultural, social, and personal factors. Menstruation-related taboos, which are prevalent in many civilizations, have an impact on girls' and women's emotional well-being, attitude, way of life, and, most significantly, health. Primary dysmenorrhea is characterized by spasmodic cramping pain in the lower abdomen that can extend to the lower back and anterior or inner thighs. Aims and Objectives: purpose of study was to investigate the effect of abdominal fascia release technique and aerobic exercises on menstrual cramps. Material and Methods: A quasi experimental research was conducted on virgin females suffering from primary dysmennorhea. The sample size of 50 was taken. The data was collected using convenient sampling. The duration of study was 2 months. WALIDD score and NPRS was used to collect the data. Results: Significance effects of both treatments using NPRS with T value 11.726 at p-value .001. A significance effect was founded between treatment using WLID with t value .003 and significance value .003. at the end it is observed that mean value of group A (aerobics)had less scale value as compared to group B(abdominal fascia release technique) so A effect is better than B. Conclusion: Primary dysmenorrhea was very common among young females. In this study we compared aerobic exercises effect with abdominal fascia releasing technique in relieving primary dysmenorrhea. Results of the study showed that aerobic exercises played a best role in relieving primary dysmenorrhea. Whereas abdominal fascia releasing technique showed minor effect in few females. So, aerobic exercises are better than abdominal fascia releasing technique in primary dysmenorrhea.

significance effect was founded between treatment using WLID with t value .003 and significance value .003. at the end it is observed that mean value of group A (aerobics)had less scale value as compared to group B(abdominal fascia release technique) so A effect is better than B. CONCLUSION: Primary dysmenorrhea was very common among young females. In this study we compared aerobic exercises effect with abdominal fascia releasing technique in relieving primary dysmenorrhea. Results of the study showed that aerobic exercises played a best role in relieving primary dysmenorrhea.Whereas abdominal fascia releasing technique showed minor effect in few females. So, aerobic exercises are better than abdominal fascia releasing technique in primary dysmenorrhea.

Prevalence of adhesive capsulitis in non-diabetic participants within age 50-70 years in Multan

Zainab Mustafa — 2024-03-31

Adhesive capsulitis includes stiffness and limitation at the shoulder joint. It is also called a frozen shoulder. Adhesive capsulitis is an inflammatory painful condition that causes a decrease in the range of motion of the joint. The healing process of a frozen shoulder takes up to two to three years most of the time.To see the prevalence of adhesive capsulitis in non-diabetic participants aged 50-70 years in Multan. Material and methods: A cross-sectional descriptive(observational) study was conducted using a self-made questionnaire. The study population was housing societies, data was collected from 278 participants from both genders, and data were analyzed through SPSS version 25. This current study showed that out of all participants, 32.01% older population were having adhesive capsulitis. The prevalence of adhesive capsulitis in non-diabetics among the older population was high.