Quantitative measurements of DNA from human saliva and optical density difference between healthy and leukemia blood
Keywords:DNA, UV/Vis spectrophotometer, human saliva, leukemia
The spectrophotometer (λ = 190-1100 nm) was used to investigate the DNA extracted from human saliva and blood of healthy and leukemia samples. Extraction buffer and Tris-acetate-Ethylenediamine tetraacetic acid (TAE) buffer were the most important buffers for extraction of DNA. Eight samples were collected from which five were for normal individuals and three were for cancer patients for the extraction and quantification of DNA. Purity of samples was checked by purification ratio (A260/A280) and most of the samples were observed to be pure. Concentration of DNA was calculated by using a relation concentration of DNA = OD at 260 nm x 50 x dilution factor. All the samples of DNA extracted in this experiment give a sufficient quantity of DNA which can be used for any diagnostic purpose. The blood samples obtained from patients with confirmed leukemia diagnosis were also investigated to correlate between absorption and optical density and their response in ultraviolet and visible region using spectrophotometer. we have related the mean value of optical density of all samples and reported how mean value vary from healthy to leukemia blood in range of ultraviolet and visible region. The analysis indicates that there were strong correlations between absorption and optical density. Healthy blood samples indicate the low transmittance of light in UV/VIS range as compared to leukemia sample.