Evaluation of Phenolic contents, Cytotoxic, and Antioxidant potential of Euphorbia nerrifolia

Authors

  • Khuram Ashfaq Department of Pharmaceutical Chemistry, Lahore Pharmacy College, Lahore
  • Muhammad Anees-ur-Rehman Ruthpfau College of Nutritional Sciences, Lahore Medical and dental College, Lahore
  • Muhammad Tariq Department of Pharmacology, Lahore Pharmacy College, Lahore
  • Nasir Ali Department of Pharmaceutical Chemistry, Lahore Pharmacy College, Lahore

Keywords:

Total phenolics, Antioxidant, Cytotoxic, Euphorbia nerrifolia

Abstract

Objective: The researchers wanted to see how cytotoxic, phenolic, and antioxidant potential of Euphorbia nerrifolia were. Methods: The organic extracts were obtained by extraction of whole dried plant with n-hexane, dichloromethane, and methanol. Phyto-chemical screening was carried out using standard methods. Total phenolics contents were measured by using the Folin–CioEcalteu reagent method. Cytotoxic action was evaluated by brine shrimp lethality bioassay. DPPH radical assay was employed for antioxidant activity. Results: Phytochemical investigations confirmed the presence of anthraquinones, saponins, cardiac glycosides and flavonoids. Highest phenolic content (276.41 ± 64 mg GAE/g dry extract wt) was observed in methanol extract. Significant antioxidant potential was exhibited by methanol extract by means of 78.8% inhibition with IC50 value of 35.71µg/ml). In brine shrimp lethality bioassay, methanol extract demonstrated prominent cytotoxicity at highest level of dose with LD50 471.05. Conclusion: The study concluded that Euphorbia nerrifolia contains significant antioxidant and cytotoxic potential. Further investigation is suggested for the isolation of secondary metabolites responsible for reported biological activities.

 

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Published

2022-03-31

How to Cite

Ashfaq, K., Anees-ur-Rehman, M. ., Tariq, M. ., & Ali, N. (2022). Evaluation of Phenolic contents, Cytotoxic, and Antioxidant potential of Euphorbia nerrifolia. International Journal of Natural Medicine and Health Sciences, 1(2). Retrieved from https://journals.iub.edu.pk/index.php/ijnms/article/view/867