Extraction and quantification of antimicrobial peptides from medicinal plants through TrisNaCl and PBS buffer

Abstract

Nature has provided plants with their own specific defence system that protect the plant from several traumatic conditions. These include environmental conditions like drought, harsh climate changes, wounding, pathogen attack and other biological as well as a biological stresses. In order to deal with all these harmful occurrences, plants synthesize a wide range of defence factors that include both primary as well as secondary metabolites. Out of these the most popular are the defence proteins which are known as antimicrobial peptides (AMP). These AMPs are actually the pathogenesis-related (PR) defence proteins.  These proteins are activated under the control of defence system of plant whenever triggered by the alarming situation. In the current study Crude protein extraction of four medicinally important plants named as Cassia fistula, Albizia lebbeck, Saccharum officinarum & Cymbopogon citratus was performen. Extraction was done in TrisNaCl and PBS buffer. Quantification of the protein content in the extract was done by Bradford assay. Concentration of protein from TrisNaCl buffer extracts was high as compared to the extracts from PBS buffer. As these proteins play their protective role in defence of the plants against pathogen attack so these extracts can better be used to check the antimicrobial activity of these plants in future to treat several infectious diseases in humans.