Determination of reference ranges of plasma glycosaminoglycans in a tertiary care centre
Keywords:Glycosaminoglycans; reference range; malignancy; ELISA
Objective: To determine reference ranges of plasma glycosaminoglycans (GAGs) in a population presenting at a tertiary care centre of Department of Chemical Pathology and Endocrinology, Armed Forces Institute of Pathology, Rawalpindi from January 2020 to December 2020.Methodology: An observational cross-sectional study which involved one hundred and twenty (120) disease-free healthy population was selected by non-probability consecutive sampling at a 90% confidence interval with a 5% margin of error. Plasma glycosaminoglycans were assayed by manual ELISA technique. The study population was stratified according to gender. Normality of data was assessed by Kolmogorov-Smirnov test. Dixon range test was employed to find outliers. After removing the outliers, mean ±, SD was calculated for plasma GAG levels (mg/L). Sex-specific reference values were determined Results: In our study, the total male population was 62 (51.7%), while the female was 58 (48.3%) in the disease-free population (n=120). The overall GAG levels were calculated as 24.12±7.9 mg/l in blood samples against the reference range of 11.48-36.76 mg/l. In males, GAG levels were found as 24.67±6.65 against a reference range of 11.67-37.7 mg/l, while in females, it was 22.22±5.36 against the reference interval of 11.71-32.73 mg/l.Conclusion: The reference range for plasma GAG was found 11.48-36.76 mg/l in our study population. GAG levels differ significantly among males and females with reference ranges of 11.67-37.7 mg/l and 11.71-32.73 mg/l, respectively.
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